Efficacy of Chitosan Immune Response Against Listeria Monocytogenes Infection in Mice

Abstract

The present research aimed to study the effect of dietary chitosan supplementation against murine
experimentally infection by Listeria monocytogenes.
forty mice were divided equally into 4 groups. The 1st and 2nd groups fed on diet supplement with chitosan
(1mg/kg diet) and (1.5mg /kg diet) for (4) weeks respectively, While 3rd and 4th groups considered as control
positive and negative groups. At (4) weeks the first three groups were inoculated intraperitoneally i/P with
(0.2) ml (1×109) CFU/ml, while the 4th group (control negative) inoculated with (0.2) sterile normal saline.
At (7) days post infection, the result revealed diet one of mice in each control positive and treated group at
(24hrs.) post infection with heavy bacterial isolation from brain, spleen and liver of infected positive group
and mild to absent bacterial isolation in the 1st and 2nd group respectively.
Grossly presence of severe congestion in the internal organs with necrotic foci seen on the splenic surface of
infected positive control while the characteristic feature in the treated infected group was hepatosplenomegaly.
Sever pathological changes were noticed in the infected positive control group characterized by suppurative
inflammation with necrosis accompanied with lymphoid depletion and amyloid like substance deposition
while the main lesion in treated infected groups showed granulomatous lesion, lymphoid hyperplasia and
mononuclear cells infiltration with heavy bacterial isolation from brain, spleen and liver of infected positive
group and mild to absent bacterial isolation in the first and second group respectively, We concluded that
chitosan stimulated and improve the immune responses in mice against Listeria monocytogenes infection.