Detection Antibodies IgM, IgG and Determination Levels of IL-33 in Iraqi Diabetic Type 2 Patients Infected with Toxoplasma Gondii

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes a disease called toxoplasmosis which

has the capability to pass its both life cycle parts asexual and sexual in the final host (cats)and also through

intermediate vertebrate hosts including humans. Diabetes mellitus is a metabolic disorder that there is high

blood sugar level over a prolonged period. This research included one hundred and twenty blood samples

that collected from diabetic patients after diagnosing them by endocrinologist at the Imamein Kadhimein

Medical City during August until the end of December 2016 with age ranging 12-76 years with mean 50.9

± 13.8. Toxoplasmosis diagnoses was occurred by using Toxo IgG and IgM antibodies immulite torch assay

while diabetes diagnosis by fasting blood sugar tests also measuring level of IL-33 in samples was done

by sandwich ELISA method that showed a group of diabetic patients infected with toxoplasmosis have

the highest level of IL-33. The results showed that a group of diabetic patients have the highest level of

glucose in diabetes test (188.31 pg/ml)while a group of diabetic patients infected with toxoplasmosis have

the highest level of IgG (106.17 IU/ml)while all samples have seronegative results for Toxo IgMalso the

highest level of IL-33 showed ina group of diabetic patients infected with toxoplasmosis (858.84 pg/ml)

while a group of diabetic patients not infected with toxoplasmosis have (556.67 pg/ml) and control group

have (315.58pg/ml).