Antitumor Activity of ?-glucan Extracted from Pleurotus Eryngii

Authors

MTT, pleurotus eryngii, Cytotoxicity, ?-glucan, Antioxidant, MCF-7, HepG2

Pleurotus eryngii, a type of edible mushroom that exhibit various pharmacological properties, including

antioxidant and anticancer effects. In the present study, extracted ?-glucan from the P. eryngii was tested as

an antioxidant and anti-tumor factor. ?-glucan was extracted and analyzed by HPLC and FT-IR. Analytical

results showed more than 90% similarity in chemical structure and purity. Potential antioxidant activity of

?-glucan was examined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and compared with ascorbic acid.?-

glucan confirmed a potential scavenging activity. The anticancer activity of the?-glucan was assessed using

different concentrations (6.25 to 400 µgmL-1) on MCF-7 and HepG2 cell lines. P. eryngii?-glucan exerted

a dose-dependent reduction in MCF-7 and HepG2 cell viability with an IC50 of 280.00 and 539.5 µg mL-1,

respectively. At the same time, no significant effect was recorded on normal cell line WRL 68. The obtained

results are expected that could be used to develop P. eryngii?-glucanas an antitumor drug

Ali Z. Al-Saffar1 , Noora A. Hadi2 , Hadeel Mohamed Khalaf3

1 Assist. Prof., 2Lecturer, 3Lecturer, Al-Nahrain University, College of Biotechnology,

Dept. of Molecular and Medical Biotechnology, Baghdad, Iraq