Preparation of Nano-Antigen for Brucella Abortus by Ultrasound Technique and Evaluate their Sensitivity by ELISA Technique
Keywords:Nanotechnology, Brucellosis, ELIZA Technology
Background: The nanoparticle technology, or nanotechnology is the science that studies the treatment of
matter on the atomic and molecular scale. Nanotechnology deals with measurements between 1 to 100
nanometers, i.e. deals with atomic clusters ranging from five atoms to a thousand atoms. They are much
smaller than bacteria and a living cell palsy.
Method: In this study, it involved the production of a new technique for diagnosing infection with Brucella
bacteria. This study included 45 samples of patients suffering from brucellosis (women and men), in addition
to 36 samples from other Brucella. All were diagnosed Injuries by hospital medical advisory staff using
various tests vitek, ELISA, API20C, Morphology, Brucella media test. The samples were examined using
the new technique compared to the routine method of diagnosis. We found that the new technique gave a
positive result to the samples infected with Brucella bacteria, while the rest of the samples without infection
with Brucella bacteria gave a negative result.
Results: It was found that the tests of Elisa and API20C, Vitek were more sensitive 100% and specialized
100% for the diagnosis of Brucella bacteria than other method morpholgy with allergic 44%and specialized
86%, Brucella mediaTest, with allergy 88 % And specialty 86%. The results of the ELISA kit test were the
nano-labs (the new technique) in this study to diagnose more successful bacteria compared to normal system
and more allergic and specialized because of the antigen-containing.
Conclusion: The new kit use in nanotechnology is more Sensitivity and Specificity for diagnosis to the
brucella, Roads routine to diagnose brucella is not effective compared with API, Vitek and ELISA .
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