Molecular and Phylogenetic Study of The H P A I (High Pathogenic Avian Influenza) Virus Sub-type H5N8 in Iraq
Keywords:Avian influenza H5N8, RT-PCR, degenerate primer, phylogenetic tree.
The Avian influenza (AI) is aspreadableinfection of the birds and humans affected by type A of influenza
virus. Highly pathogenic avian influenza (HPAI) caused by the viruses subtype (H5N1) and (H5N8) is
consider as a definite risk to the poultry industry and human health and it is started as the occasional
revolutionize a satisfactory because of migratory bird which consider as a carrier of the disease. Twenty
oropharyngeal swabs were collected from infected flock have suffer from sudden death with high mortality,
all these samples were collected from (1-5) days after clinical signs appears and the results summarized by
three farms infected with HPAI subtype (H5N8), three viral isolates strain out of 20 samples were identified
by RT-PCR assay, two of them have layer origin while one have broiler origin, the three sequencing results
in this study have been presented to the gene bank database under accession number MN689674.1 and
MN658854.1, phylogenetic analysis of our sequences were used to relatedness of strain obtaining with other
avian influenza viruses around the world, the a current sequences of avian H5N8 strains were found to be
related as a common ancestor with Iranian registered strain.
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