Nanocurcumin Protective Effect on Lipid Peroxide of Lead Acetate Induced White Rats (Rattus norvegicus) Ovarian Granulosa Cells

Abstract

Objective: This study aims to investigate the protective effect of nanocurcumin on lipid peroxidation of rat
ovarian granulosa cells induced by lead acetate (Pb).
Methods : A total of 45 mice were divided into a negative control group (C-) receiving corn oil, a positive
control group (C+) receiving corn oil and lead acetate (Pb) 40 mg/kg bw/day, experimental group 1 (E1),
experimental group 2 (E2), experimental group 3 (E3) receiving 50, 100 and 200 mg/kg/day nanocurcumin
dissolved in corn oil and lead acetate (Pb) 40 mg/kg bw/day. The treatment was given orally and the treatment
time was daily at 08.00 AM for 26 days. Ovaries were removed for immunohistochemical analysis of MDA
expression.
Result : The positive control group had the highest MDA expression of ovarian granulosa cells (94.2 ± 10.5)
and was significantly different (p <0.05) from negative control group (1.5 ± 1.4), experimental group 1 (17.4
± 10.5), experimental group 2 (11.4 ± 8.5) and experimental group 3 (7.2 ± 4.0).
Conclusion : Nanocurcumin as a protective oxidative stress on ovarian granulosa cells due to exposure to
lead acetate, by protecting lipid peroxidation.