Artemesiaannua L. Silver Nanoparticles Exhibit Anticancer Activity by ROS mediated apoptosis in MDA-MB-231 Cells

Abstract

Aim of this study was to investigate the growth inhibitory effect of Artemesisannua L. silver nanoparticles
(A.annuaAgNPS) on human triple negative breast cancer tumor cell line MDA-MB-231. Cells were
cultured and treated with A.annua water extract and A.annuaAgNPS to determine the half inhibitory
concentration (IC50). Oxidative stress was determined using lipid peroxidation (LPO), ROS generation and
by determining the levels of antioxidants, superoxide dismutase (SOD) and reduced glutathione (GSH).
Further, the mechanism of apoptotic induction was assessed by DNA fragmentation analyses. We observed
that A.annuaAgNPS significantly caused growth inhibition by increasing levels of LPO and ROS, in a dose
dependent manner with reduced level of endogenous antioxidant enzymes. Increased shear in genomic DNA
from MDA MB231 cells treated with A.annuaAgNPS shows apoptosis to be the cause of cell cell death. We
conclude that A.annuaAgNPS can be useed as a therapeutic agent against breast cancer.