Detection of the Genetic Diversity and the DNA of Some Species of the Cruciferous Family (Brassicaceae) using the RAPD-PCR Markers

Abstract

The current study aimed to uncover the genetic diversity of eight local species of the Brassicaceae plants
by using RAPD markers, where the genetic relationship was determined, the genetic dimension was found
between the studied species and the genetic finger print of some of these species was identified. (DNA)
was extracted from the young leaves of the cruciferous family plants using the method Ready (Kit). The
concentration of the extracted DNA ranged from 148.35-165.3 ng /µl with a purity of 1.53- 1.82. The results
obtained from the study showed that 307 band were all differentiated (100%), higher in number than bands
(38) with (12.38%) in the primer (MQ10), lower in number than bands (21) and with (6.84%) in the primer
(MQ6). The highest molecular volume (pb2500) was characterized by (B1) in the primer (MQ7), while the
less Molecular volume (100bp) in the two species (B3 and B5) was distinguished by the primer (MQ8). The
results of the genetic dimension showed that the lowest value is for the genetic dimension (0.260) appeared
between the two species (B2 and B3), and the highest value of the genetic dimension was (0.975) appeared
between the two types (B4 and B5). The phylogenetic tree was drawn between the studied species and the
species were divided into two major groups. The first group included the species (B1, B2, B3 and B4) and
the second group included the species (B5, B6, B7 and B8).