Synergistic Effect of Amikacin and Ciprofloxacin on pelA and algD Genes in Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa is one of the most important pathogen in both hospital and community-acquired
infections, it is a classical opportunistic pathogen, and this organism does not normally attack healthy
tissue, and rarely causes infection in the general population. It is possess various virulence mechanisms
to adhere, colonize and biofilm formation. Samples were collected from different hospitals in Baghdad,
samples were collected and categorized in to two main groups: eighty clinical samples and forty as an
environmental samples. Forty one isolates were identified as P. aeruginosa by using conventional methods
and confirmed by VITEK-2 compact system, biofilm were detected by three methods: congo-red agar, tube
method and microliter plate. Synergistic Effect of Amikacin and Ciprofloxacin on biofilm formation were
detected phenotypically by using microliter plate and genetically by Real-time PCR for pelA and algD
Genes which are responsible for biofilm formation. The results shows an obvious differences in biofilm
formation phenotypically and genotypically for the treated isolates