Variation of 16SrRNA gene for Some Nosocomial Bacteria Isolated from Ramadi Teaching Hospital for Women and Children

Abstract

The aim of this studsy is to diagnose bacterial strains in hospitals by the 16SrRNA gene and to know the
variation of the nucleotide sequence. The study of bacteria in hospitals is of great importance to identify the
extent of change in these species and their resistance to antibiotics. This indicates that these bacteria have the
ability to change or obtain new variants to be able to resist antibiotics. In this study, samples were taken from
different locations of the Ramadi Teaching hospital for women and children and were cultured on different
media. They were also stained and examined under light microscopy and some biochemical tests were
conducted. Pure colonies were obtained after re-culture and then extracted the DNA from the 16 isolated
samples and measuring its concentration. Using the specific primer for 16SrRNA genes, the samples were
amplified by a PCR and the size was shown to be 1500 bp. The unknown bacteria were sent to the sequence.
Some samples showed variations in comparison with the data presented by NCBI. This is evidence of some
variations shown by bacteria to overcome antibiotics and increase their severity and spread in hospitals,
whether in kitchens, emergency or preterm infants, as well as laboratory staff and lab equipment.