Phylogenetic Tree Analysis of First Psychrobacter Sp. Strain From Blood of Iraqi Patient; A Case Report

Abstract

Psychrobacter spp. are a Gram negative bacteria, aerobic, non-motile, small, with coccobacilli shape.
Originally isolated from seaweed samples and marine environments. Recently considered as rare
opportunistic human pathogens. Sixty –five years old women admitted to hospital with diabetic mellitus
and stage 4 pressure ulcers (PU) with seizure and mild fever 37.9 °C. A gram staining of blood culture
revealed gram negative bacteria have a cocobacilli shape. The VITEK2 system (bioMérieux) misidentify the
isolate as Acinetobacter bumannii complex with low discrimination. The submission of the bacterial isolate
to the GenBank BLAST search tool revealed that the Iraqi isolate show 100% homology with Psychrobacter
sp. From china with accession number ID: MK205167.1, the next matches with Uncultured Psychrobacter
sp. ( ID: KF859544.1 China) Psychrobacter pulmonis(ID: KU364058.1, India), Psychrobacter pulmonis
(ID: MH550129.1, China)with 99% similarity for each one. This Psychrobacter sp. was the first isolate from
bacteremia patients in Iraq. The identification based on 16S rRNA gene sequence for precisely identify this
bacteria that misidentified by VITEK2 system