Using Paper Waste and Local Bacterial Isolates to Produce Biomass and Cellulase Enzyme- A Study Waste Recycling

Abstract

The study aims to isolate the cellulose-degradation bacteria that produce the cellulase enzyme as well as the
production of SCP protein from Ramadi city soils and to obtain efficient local bacterial isolates in production
and efficient isolation was selected in the production of cellulase enzyme and single cell protein which is
Bacillus subtillis bacteria tested the efficiency of the isolated isolation to produce the enzyme and single cell
protein. The cell using local media contains the paper waste available in the environment and contaminated
with it. Initial transactions were made for this paper waste and these factors included (heating to boiling
point, dilute sulfuric acid and dilute sodium hydroxide). The paper waste used included the white cardboard,
yellow cardboard and white paper Which was used as a single source of carbon and energy, after testing
the waste paper used, it was found that the white cardboard treated with heating gave the best production of
the cellulase enzyme and single-cell protein. The bacterial isolation used showed the best growth and gave
the highest production of the enzyme and the single cell protein at pH 7, while the best temperature for the
production of the enzyme was 45 ° C. The optimum temperature for the production of the single cell protein
was 40 ° C. As for the carbon concentration, which is the white cardboard treated with heating, it was better
to produce than Concentration is 2%. As for the production of single cell protein, the highest output at
concentration was 4%. The best bacterial vaccine size for enzyme production was 3 ml / 100 ml medium. As
for the production of single cell protein, the size of the inoculum gave 1.5 ml / 100 ml the best production.
The addition of the organic nitrogen source (urea) gave a clear increase in enzyme production and single
cell protein, and when adding 0.5 g urea / 100 mL medium. Finally, the results showed that the best period
duration to obtain the highest cellulase enzyme production was after 96 hours, while it was found that the
best production of single-cell protein was after 24 hours incubation.