Molecular Investigation of fliC and blaTEM1 Gene among Clinical Isolates of Salmonella Typhi

Abstract

This study aimed to isolate and diagnose Salmonella typhi by flic gene and investigated the presence of
blaTEM1 gene by using PCR technique and electrophoresis systems in Najaf Province includes 40 clinical
specimens blood for both sexes with an age ranged between )5-60) years old .The study result by using a flic
gene was detected in 30 of the 40 isolates of S. typhi isolated from typhoid patients , which was conducted in
the diagnosis of bacteria concluded that the technique PCR are the most sensitive in the detection of S. typhi
compare with other way, blaTEM-1 was detected in 16 of the 40 isolates from S.typhi . The conseder the bla TEM
gene encoding TEM-1 b-lactamase is think to give resistance only to penicillins and early cephalosporins;
thus , the spectrum resistance of TEM-1 descendants may extend to second, third and fourth-generation
cephalosporins.