CD14 C(-260)T Polymorphism and Blood Levels of the Soluble Endotoxin Receptor CD14, their Association with Risk of Ischemic Stroke in Iraqi Populations

Abstract

Background: Stroke is a main cause of death and adult long-term disability worldwide, and stays as an
enormous burden for society due to lack of illustrated etiology and effective treatments. Aim: the present
study was conducted to investigate the association of CD14 genotype and plasma levels of soluble(s) CD14
with risk of ischemic stroke. Methods : A total of 40 patients with ischemic stroke were included in the study
who were admitted to hospital from the period between March to August 2019, and other groups consist
of 40 apparently healthy individuals. A five ml of blood samples were collected, 2 ml of each sample for
polymerase chain reaction amplification and detection of CD14 technique. The remaining (3ml) for CD14
monocyte count by Flow cytometry technique. Results: CD14 C(-260)T genotype was not significantly
associated with increased risk of IS (P= 0.367). However, sCD14 plasma levels were higher in subjects with
TT genotype compared with those with CT or CC genotype (P= 0.013). The count of CD14 Monocytes,
in Ischemic Stroke patients was significantly higher than that of healthy control group. Conclusion: a
significant correlation between CD14 genotype and sCD14 levels were higher level in subjects with TT
genotype compared with those with CT or CC genotype.