Spectrophotometric Determination of Ampicillin with Sulfanilic Acid by Oxidative Coupling Reaction

Authors

  • Samar Ahmed Darweesh1 , Hamsa Muneam Yaseen2 , Ruaa Muayad Mahmood3 , Rokayia Samir Al-Khalisy4 , Husam Saleem Khalaf5

DOI:

https://doi.org/10.37506/ijfmt.v14i4.11844

Keywords:

Spectrophotometric determination, Ampicillin, Sulfanilic acid, Oxidation reaction.

Abstract

Sensitive, economic, simple and accurate spectrophotometric method for estimation of ampicillin in bulk
and dosage form is described. Ampicillin was oxidized by sodium hypochlorite and coupling with sulfanilic
acid in the presence of sodium hydroxide to obtain a stable yellow colored chromogen which exhibit a
maximum absorption (?max) at 400 nm. The optimum conditions were carefully evaluated .
Plot of absorbance against concentration was linear over the range (50-300 µg.ml-1). This method was applied to
the estimation of ampicillin in pure drug and commercial formulations successfully.

Author Biography

  • Samar Ahmed Darweesh1 , Hamsa Muneam Yaseen2 , Ruaa Muayad Mahmood3 , Rokayia Samir Al-Khalisy4 , Husam Saleem Khalaf5

    1
    Lecturer/ Chemistry Department / College of Education for Pure Science- Ibn Al-Haitham /University of Baghdad/
    Iraq, 2Assist. Prof. Chemistry Department / College of Education for Pure Science- Ibn Al-Haitham /University of
    Baghdad/Iraq

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Published

2020-10-29

Issue

Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology

Section

Articles

License

https://creativecommons.org/licenses/by-nc/2.0/deed.en

How to Cite

Spectrophotometric Determination of Ampicillin with Sulfanilic Acid by Oxidative Coupling Reaction. (2020). Indian Journal of Forensic Medicine & Toxicology, 14(4), 2016-2021. https://doi.org/10.37506/ijfmt.v14i4.11844