Genotypic, Phenotypic Identification and Antibiogram Assay of Mrsa Isolated from Healthy Individuals in Wasit Province

Abstract

Staphylococcus aureus is a Gram-positive, facultative anaerobic, non-spore-forming, coagulase -positive
bacterium. the Methicillin resistance is mostly mediated by the presence of mecA gene acquisition of mecA
gene leads to altered penicillin binding protein (PBP), called PBP2a, with low binding affinity to methicillin.
A total of 52 S. aureus strains were analysed. Antibiogram assay for 52 healthy carriers of MRSA isolates by
using Vitek 2 system to examine 16 different antibiotics.Methicillin resistance was confirmed by presence
of the mecA gene by PCR. Of 52 S. aureus samples, 52 isolates of them were MRSA confirmed by targeting
mecA gene using PCR. Aims of this study detect methicillin resistant Staphylococcus aureus from nose of
healthy individuals in Wasit province and confirming that by PCR to detect mecA Gene.