Molecular and Phenotypic Detection of Some of the Coded Genes for Virulence Factors in Mrsa and Mssa Isolated From Different Clinical Cases

Abstract

The current study included the collection of 100 clinical samples obtained of patients reviewed in
the laboratories of Ramadi hospital for women and children and Hit General Hospital and some civil
laboratories within the city of Ramadi, including Bacterimea samples, Urine, abscesses, heart inflammation,
wounds, rhinitis, osteoporosis, otitis ,Laryngitis, sputum (35, 20, 5, 6, 7, 5, 8, 7, 4, 3) samples respectively
to investigate the presence of staphylococcus aureas . The results of diagnosis of bacterial isolates showed
that there were 55 bacterial isolates belonging to the S.aureus bacteria after they were grown on the culture
media and examined microscopic as well as the use of some of the tests on the chemical, and the diagnosis
was confirmed using the Vitek2-compact system .The diagnostic results were confirmed by the use of
genetic diagnosis. The results of the phenotypic investigation were identical to the results of the genetic
investigation using the polymerase chain reaction technique in terms of possessing isolates producing both
hemolycin , on the HlA encoding genes for the production of type and hlB encoded for type
production, For plasma blood on the co-a gene, and the resistance isolates of methylation on the mecA gene.