Investigation Study of Human Spermatozoa Proteome

Abstract

The current study has been conducted on health human sperm to identify the different types of amino acids
and entire proteins of sperm. Two methods have been applied to characterise of human sperm, firstly extract
lysate of sperm, then digest and run it in mass spectrometry, secondly, total sperm lysate was run in SDS
page gel and bands were stained with coomassie, then band was cut and digest to extract of whole amino
acids and also was run in mass spectrometry. Result revealed that sperm have several proteins, which were
involved with different binding proteins, soluble proteins, and glycoproteins, transmembrane proteins.
Current result was displayed that mass spectrometry can be used to recognise data of proteins of sperm
and detected types of it. This technique was applied on whole lysate and gel bands of sperm and created
database of sperm proteins which can be investigated and consider more interesting for researchers. This
study is concluded that human sperm have important transmembrane, enzymes, glycosylated proteins,
glycoproteins and uncharacterised proteins which might be answered many theoretical aspects of biological
and biochemical sperm migrations before and after fertilization.