Detection of B1 Gene in Toxoplasma Gondii and the Role of Interleukin 2 in Abortive Women Infected by this Parasite

Abstract

The study included 80 blood and serum samples of aborted women with Toxoplasma gondii infection
and healthy, uninfected and non-aborted women in the control group. For the period from August-2018
to June-2019, in collaboration with the Falluja teaching hospital for women and children and the Ramadi
teaching hospital for women and children, the Enzyme-Linked Immunosorbent Assay (ELISA), also to the
Toxoplasma gondii (B1) gene, the Nested polymerase chain reaction technique are used. The results of the
study showed by that Elisa Toxoplasma gondii, IgG infection (50%), IgM infection (12.5%), and IgG & IgM
Infection (12.5%), were mean (108.16±47.02) (99.02±31.47) (96.45±20.68) respectively compared with the
control group (25%) (0.94±0.93) and Interleukin-2 shown in IgG group (48.66±12.01) and control group
(14.28±6.84) decreased compared to IgM (81.19±9.66), and IgG & IgM (80.71±9.18) group. Nonetheless,
using nested PCR, the B1 gene showed just (12.5%) of all study samples in IgM and IgG&IgM alone and
all IgG negativly