Blood Lymphocytes Detection in Iraqi Diabetic Type 2 Patients Infected with Chronic Toxoplasmosis by Using Flow Cytometry

Abstract

Toxoplasma gondii is an obligate parasite infects different species of mammals as intermediate hosts while
infects number of family of cats as definitive hosts. It has three morphological types: tachyzoites, bradyzoites
and sporozoites. It causes (H.I.) humoral immunity and (C.M.I) cell mediated immunity response which is
measured one of the most characteristic immunological types of this parasite infestation. Diabetes mellitus
(DM) is considered as a heterogeneous group of metabolic abnormalities with hyperglycemia due either to
a reduction of insulin biological function or absolute insulin deficiency. Researchers discovered that long
term infection with T. gondii may be dangerous agent for DM type 2, and there is no relation between
toxoplasmosis and DM type 1. The aim of this study include detection of CD3 in Iraqi diabetic type 2
infected with chronic toxoplasmosis by using flow cytometry. This study included 100 blood samples were
collected from Iraqi diabetic type 2 patients and 50 blood samples of healthy volunteers with age range
(15-75) years and mean 49.9±12.8 from Al – Imam Ali general hospital during May 2018 until August in
the same year. Diagnose of toxoplasmosis infection was done by using rapid diagnosis test/ cassette and by
Toxo IgG antibodies immulite torch assay while diabetes diagnosis by fasting glucose tests in addition CD3
were detected by using flow cytometric method. Diabetic patients a group infected with toxoplasmosis have
the highest level of blood blood sugar in comparison with other groups. Forty – five samples of diabetic
type 2 infected with chronic toxoplasmosis by detection IgG antibody in highly level 106.2± 90.2 with
highly significant differences in comparisons with other groups ( diabetic patients 3.6± 1.2 and control group
4.0±0.6 ) while any results for IgM Abs didn’t found in this study. Seventy – five samples studied in flow
cytometry method which include twenty – five samples for each group. Diabetic patients with toxoplasmosis
recorded highest CD3 its mean 30.7± 14.51 pg /ml flowed by diabetic patients group has 24.84±9.47 pg /ml
and control group has 20.03± 9.42 pg /ml with significant differences.