Molecular Characterization of Selected Genera of the (Apiaceae) Family using SSR Molecular Markers

Authors

  • Raad Al-Taif Jassim1 , Ashwaq Talib Hameed2

DOI:

https://doi.org/10.37506/ijfmt.v14i4.11905

Keywords:

canopy plants (Apiaceae), multiple shapes, genetic study. Corresponding author: Raad Al-Taif Jassim [email protected] Introduction Canopy plants (Apiaceae) is one of the largest plant groups which carries an economical importance which are used as spices like (cumin, fennel, coriander etc…) and vegetables like (celery, carrots, dill and parsley) and medical crop regardless of the taste and flavor that it adds to food, moreover its high oil content , antioxidants, minerals and vitamins that are important to our health, which makes it a potential treasure for neutral materials (1) and it contributes in peoples everyday life in an essential way like celery, parsley, cumin, anise, carrot and dill (2) and several more add to wild species.

Abstract

The conducted study which included the study of a few selected races from canopy plants from the chemical
perspective which included Apium graveolens L, Anethum graveolens L, Coriandrum sativum L, Petroselium
crispum L, cyminum Cuminum L, Foeniculum vulgare L, Pimpinella anisum L, Daucus carrot L and Ammi
majus L. The DNA was detected and extracted and Conducted molecular tests on the DNA using (SSR
Simple Sequence Repeats) using the polymerization device (PCR) and electrophoresis process on Agarose
Gel.
Five selected and specific prefixes were used which approved its effectiveness in giving multiple shapes
(Polymorphic) among the studied plants of 95%, as packs were obtained from a total number of 14 out of
53 different pack with an arithmetic average of 2.8 for each prefix. Cluster analysis and phylogenetic tree
have shown that there is a genetic affinity between 0.84615-0.125 according to the two variables data (1-0)
the genetic similarity using cluster analysis (UPGMA)has allowed to draw the portfolio diagram among
the studied races, It can be said that the SSR Simple Sequence Repeats can be an effective technique for
studying molecular characterization and can be used to separate the genetic relations in the plant world.

Author Biography

  • Raad Al-Taif Jassim1 , Ashwaq Talib Hameed2

    1
    Post graduate/ Anbar University of Anbar / College of Education for Pure Sciences / Department of Biology/Iraq,
    2
    Assist. Prof. Dr. Anbar University of Anbar/ College of Education for Girls / Department of Biology/Iraq

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Published

2020-10-29

How to Cite

Molecular Characterization of Selected Genera of the (Apiaceae) Family using SSR Molecular Markers. (2020). Indian Journal of Forensic Medicine & Toxicology, 14(4), 2326-2332. https://doi.org/10.37506/ijfmt.v14i4.11905