Sensitive Simultaneous Estimation of Atorvastatin Ca in Pure and Dosage Forms Via Developed CFIA using 1,2 Naphthoquinone-4-Sulfonate as a Suitable Organic Agent

Abstract

Objectives: A sensitive visible spectrophotometric method and FIA/merging zones technique was developed
for the determination of atorvastatin calcium in pure material and tablet dosage form.
Methods: Atorvastatin calcium has a free carboxylic moiety in its structure, which when being deprotonated
in basic medium facilitates associated the reagent with the drug. This method was based on the formation
of red colored chromogen of drug with 1,2-Naphthaquinone-4-sulfonate(NQS) in basic medium (NaOH).
The absorbance of the chromogens was measured at their respective wavelengths of maximum absorbance
against the corresponding reagent blank
Results: The red colored product is directly completed in basic medium and exhibits maximum absorption
at 525 nm. Different factors affecting the formation of the product and optimized in order to obtain the best
conditions for the experiment and its stability were studied. Method validation was done over a concentration
range of 2-10 and 1-20 ?g/mL for batch and FIA method respectively.