Low Carbohydrate High Protein Diet Reduces Levels of Tumour Necrosis Factor-? (TNF-?) in Rats (Rattus norvegicus)

Authors

  • Hany Aryani1,2, Budi Santoso3 , Bambang Purwanto4

DOI:

https://doi.org/10.37506/ijfmt.v14i4.12034

Keywords:

low-carbohydrate-high-protein diet (LCHP), polycystic ovary syndrome, and TNF-? levels

Abstract

The purpose of this study is to explain the effect of a low-carbohydrate-high-protein (LCHP) diet on
reducing TNF-? levels. The study was carried out with true experimental post-test only control design, using
3-month-old females rats (Rattus norvegicus) with a bodyweight of 200-300 g, which were divided into
three groups: Negative control (K-) (rats without treatment were fed with broiler standard feed), Positive
control (K+) (PCOS-IR model rats were fed broiler standard feed), and Treatment (P) (PCOS-IR model
mice were fed with LCHP diet). Serum taken from the rats’ heart on day 49 was examined for TNF-? levels
using Bioassay Germany ELISA kit. Statistical analysis was carried out using ANOVA and Post Hoc tests.
ANOVA analysis of TNF-? resulted in a p value of 0.0017, indicating significant difference of TNF-? levels
when LCHP diet was applied, followed by Post Hoc test found that the TNF-? level of the treatment group
(p = 0.006) is lower than the positive control group (p = 0.038). One of the roles of a low-carbohydrate diet
in the pathogenesis of PCOS-IR is to provide an inflammatory repair effect by increasing insulin sensitivity,
improving insulin levels, and stimulating an increase in steroidogenesis synthesis. Increased breakdown of
fatty acids is inhibited, so that it does not cause a build-up of fatty acids and does not induce an increase
in proinflammatory cytokines against gonadotropins, increasing FSH secretion and effecting follicular
development.

Author Biography

  • Hany Aryani1,2, Budi Santoso3 , Bambang Purwanto4

    1Doctoral Student, Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Airlangga, Surabaya,
    Indonesia, 2
    Lecturer, STIKES Husada, Jombang, Indonesia, 3
    Professor, Department of Obstetrics and Gynecology,
    Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia, 4
    Lecturer, Department of Physiology, Faculty of
    Medicine, Universitas Airlangga, Surabaya, Indonesia

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Published

2020-10-29

How to Cite

Low Carbohydrate High Protein Diet Reduces Levels of Tumour Necrosis Factor-? (TNF-?) in Rats (Rattus norvegicus) . (2020). Indian Journal of Forensic Medicine & Toxicology, 14(4), 2923-2928. https://doi.org/10.37506/ijfmt.v14i4.12034