Low Carbohydrate – High Protein Diet Does Not Provide Positive Effect on P38 Mitogen-Activated Protein Kinase (p38MAPK) Expression

Authors

  • Hany Aryani1 , Budi Santoso2 , Bambang Purwanto3

DOI:

https://doi.org/10.37506/ijfmt.v14i4.12035

Keywords:

insulin resistance, p38MAPK expression, polycystic ovary syndrome.

Abstract

P38 mitogen-activated protein kinase (p38MAPK) is the activity of the p38MAPK pathway in muscle cells
causing mitogen-activated disorders that are responsive to stress stimuli, such as cytokines, and involved
in cell differentiation, and apoptosis. Characteristic of p38MAPK expression contributes to metabolic
signal on the reduction and excessive production of ovarian androgen found among women with endocrine
and metabolic disorders. We suspect that p38MAPK expression changes in ovarian granulosa cells. This
research aims to observe changes in p38MAPK expression among PCOS-RI mice fed by a low-protein,
high-carbohydrate (RKTP) diet. Testosterone propionate was given to induce the PCOS-IR model, 0.1
ml injection of for 28 days intramuscularly on 18 mice. Each mouse was examined for vaginal swabs
and ovarian histology. At the end of treatment, the p38MAPK expression score kept increasing and had a
significant negative effect with ANOVA test p value = 0.149 (p >0.05). The results of the treatment group
posttest showed p38MAPK expression, and there was a decrease compared to the control group. These
occurred because the RKTP diet has not been able to stimulate RE stress through the NFK? pathway, so
the NFK? pathway increased AGEs receptors on granulosa cells that played a role in oocyte maturation as
a response in the follicular development process on PCOS-IR model. Moreover, in this research RKTP diet
had not been able to reduce p38MAPK expression in granulosa cells in the ovary

Author Biography

  • Hany Aryani1 , Budi Santoso2 , Bambang Purwanto3

    1Doctoral Student, Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Airlangga, Surabaya,
    Indonesia, 2Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga,
    Surabaya, Indonesia, 3Professor, Department of Physiology, Faculty of Medicine, Universitas Airlangga,
    Surabaya, Indonesia

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Published

2020-10-29

How to Cite

Low Carbohydrate – High Protein Diet Does Not Provide Positive Effect on P38 Mitogen-Activated Protein Kinase (p38MAPK) Expression . (2020). Indian Journal of Forensic Medicine & Toxicology, 14(4), 2929-2933. https://doi.org/10.37506/ijfmt.v14i4.12035