The Anti-Proliferative Activity of Ephedra Aphylla Methanol Extract Against Liver Cancer Cell Line

Abstract

This study aimed to investigate the anti-proliferation activities of methanol extract ephedra aphylla against
(HC) liver cancer cell line in vitro and to identify the possible mechanism of action. Anti-proliferative
activity of the plant extracts against the cancerous cell lines was tested by 3-(4, 5-dimethylthiazol-2-yl)-2,
5-diphenyltetrazolium bromide (MTT) assay on liver cancer cell line (HC). Gene expression studies using
qPCR for the proapoptotic gene P53 were also carried out. The methanol extract of ephedra aphylla exhibited
promising anti-proliferative activity. Dose response relationship was shown after 72hr incubation. Significant
cytotoxic activity of methanol extracts has identified on HC, IC50 was (6.79 ?g/ml). Furthermore, methanol
extract significantly up-regulated the expression of P53. Thus, these findings suggest potential utilization of
ephedra aphylla as new source in researching for new anticancer agents.