Characterization of Aflatoxin B1 Biomarkers as Preference for Antitoxin Candidates for Biosensors for Halal Food Supply

Abstract

The fungus grows on various types of food and feed, its growth will cause damage, including damage to
flavor, color, softening, and the formation of toxic compounds. The damage is because the fungus produces
extracellular enzymes that break down certain compounds in food and feed, and produce toxic secondary
metabolites, called mycotoxins. Fungal contamination requires serious attention, not only because it causes
damage to food and feed but is related to the potential of the fungus to produce mycotoxins and form
conidia that are pathogenic or allergic, mycotoxins produced by various types of fungi, each of which has
varying toxicity, in general chronic causes mycotoxicity. Mycotoxins have caused several types of diseases
in humans and animals. Aflatoxin B1 (AFB1) is a potential toxin as a hepatocarcinogen. Eating foods tainted
with AFB1 can cause acute (short term) and chronic (moderate or long term) poisoning; chronic disorders
such as disorders of the central nervous system, cardiovascular and lung systems, and digestive tract. Some
mycotoxins are carcinogenic, estrogenic, mutagenic, teratogenic, and immunosuppressive. The purpose of
this study is the characterization of Aflatoxin B1 Biomarkers as Preference for Antitoxin Candidates for
Biosensors for Halal Food Supply. The method of this research is AFB1 induction in mice, observed for 14
days, on the 14th day liver organs were taken. The liver is prepared into organ extracts, analyzed using IHC.
From the research results obtained protein expression in the liver organs of experimental animals by the IHC
method. Further research is needed as an antitoxin and biosensor.