The Effect of 2-Thioxo Imidazolidin-4-Ones (2- Thiohydantion) on Anticancer Activity: An in Vitro Study

Abstract

Background and Purpose: Cancer is one of the most common factors of death in society today. Despite
much research into cancer and its treatment, it is still one of the greatest health problems. The biggest
obstacle to cancer treatment is that they are inherently resistant to chemotherapy or become drug resistant
during treatment. For this reason, researchers around the world have made great efforts to identify new
natural or synthetic compounds with anticancer properties. So the aim of this study is to find out the effect
of 2-Thioxo imidazolidin-4-ones (2-Thiohydantion) on anticancer activity.
Materials and Methods: Imidazoles are compounds that have antibacterial, antifungal and antitumor
properties in many studies. Through the results of this study, we discovered effectiveness of the new
compound of 2-Thioxo imidazolidin-4-ones (2 Thiohydantion) as an anticancer agent against a cell line that
is a cancer model system. To determine the IC50 of this compound, we treated the cancer cells by different
concentrations (10, 20, 30, 40, 80 and 160 ?g / ml) and then the cells were evaluated by MTT assay after 24
and 48 hour intervals.
Results: After preparing the required compound, MTT assay showed that the IC50 of 2-Thioxo imidazolidin4-ones (2-Thiohydantion) compound on MCF7 cells, 135 and 40 ?g / ml, respectively, after 24 and 48 h,
respectively. In addition, the toxicity of these compounds on normal cells was evaluated, which is very
different from their toxicity for cancer cells, and this is a very important feature. 2-Thioxo imidazolidin-4-
ones (2-Thiohydantion) has the ability to stimulate cell death in cancer cells, and these results provide new
insights into the use of these compounds in cancer treatment.
Conclusion: So 2-Thioxo imidazolidin-4-ones (2-Thiohydantion) at different concentrations significantly
reduced the growth of cancer cells compared to the control group (50% inhibition of cell growth). Future
studies will need to investigate the mechanism of this cytotoxicity as well as the possible anticancer
effectiveness of this compound in vitro and in vivo.