The Protective Effect of Annona Extracts on Renal Histopathology Induced by Ethylene Glycol in Male Rabbits

Abstract

Annona is one of the many plant extracts that have been explored owing to their
anti-inflammatory and anticancer effects. Male locale rabbit were separated into five groups, Group I
consider as control, The 4th other groups were treated orally with 0.75% ethylene glycol (EG) to induce
renal damage this treatment done till 30th days. Group III to V animals were served as curative regimen
and received flavonoids, glycosides and alkaloids extract of the plant of Annona at a dose of 100 mg/kg
body weight from 15th day to 30th day. The extracts was administered twice daily by oral route. significant
kidney injury in EG animals as demonstrated by a significant elevation (P= 0.05) in serum activities urea,
creatinine, total protein and calculated globulin levels. Meanwhile there was significant drop (P=0.05) in
albumin, and A/G ratio compared to the control and treated group , The levels of lipid peroxidation were
significantly higher in the Serum of the groups of animals treated with EG , while the levels of SOD,
CAT and GPx were significantly lower than animals were treatment of EG group with Annona significantly
elevated the antioxidant mechanisms differ with that before treatment. Renal Histopathological changes
includes deposition of oxalates in the renal tubules lumen, degeneration and necrosis of epithelium of renal
tubules , congestion of blood cells , infiltration of inflammatory cells and atrophy in glomerular tuft leading
to expansion of bowmans spaces. While Histopathological changes in animals group treated with Annona
extract and ethylene glycol were limited dilatation of renal tubules with vascular changes with blood vessels
congestion and interstitial hemorrhage , reduced renal damage revealed by less degenerative and necrotic
lesion in tubular epithelium and no inflammatory reaction and the glomeruli appeared normal compared with
ethylene glycol sections.