Biological Hazard on Multidrug Resistance (MDR) of Escherichia Coli Collected From Cloacal Swab of Broiler Chicken on Wet Markets Surabaya

Abstract

Objective: The purpose of this study was to determine the multidrug resistance (MDR) profile of Escherichia
coli from broiler chicken from Wonokromo Market and Tambahrejo Market Surabaya to some antibiotic
groups, namely streptomycin, levofloxacin, cefotaxime, trimethoprim and chloramphenicol.
Materials and Methods: Seventy samples were taken from Wonokromo market and Tambahrejo market,
each of which had thirty-five samples. Sampling was used cloaca swab technique. Then inoculated on Eosin
Methylene Blue Agar (EMBA) media, purification of bacteria on EMBA media, and tested biochemically
with Sulfide Indol Motility (SIM) and Triple Sugar Iron Agar (TSIA) media.
Antibiotic sensitivity test was used the Kirby-Bauer method. Muller-Hinton Agar (MHA) media incubation.
Then the inhibition zone was measured according to the Clinical and Labortary Standard Institute (CLSI)
standards. Detect positive multidrug resistance (MDR) bacteria characterized by resistance to ?3 types of
antibiotics.
Results: Detection results of Escherichia coli multidrug resistance (MDR) at Wonokromo market was
85.7%, and at Tambahrejo market was 51.4%. Presumptive Extended Spectrum Beta Lactamase (ESBL)
producing E. coli at Wonokromo market was 14.3% and at Tambahrejo market was 2.9%.
Conclusion: The high level of MDR of Escherichia coli from cloacal swabs of broiler chicken was a threat
to public health and the environment, and is an important concern to reduce the rate of its spread