The Possible Nephroprotective Effect of Roflumilast Ameliorating Glycerol -Induced Myoglobinuric Acute Renal Injury in Rabbits Model

Abstract

urther leads to tissue necrosis and inflammation. While, roflumilast N-oxide is an active metabolite of
roflumilast that rising the cyclic adenosine-3?, 5?-monophosphate by inhibiting PDE4.
Thirty rabbits were divided into three groups, control group was received normal saline intra-muscularly and
drived from water for eighteen hours before. Second group was received a single dose glycerol injected intramuscularly after drived from water for eighteen hours .Third group was received roflumilast intraperitoneal
daily for three days after received a single dose of glycerol .
All animals were sacrificed after 72 hour, and then blood collected by cardiac puncture for evaluation the
changes in the levels of renal function tests and histopathological study of rabbit’s kidneys tissues are
evaluated evaluation and scoring the changes of tested agents. The numerical data are expressed as means ±
standard error means and analyzed by using SPSS version 20.
The results of the levels serum urea and creatinine found to be significantly reduced by roflumilast using
ANOVA test. Besides, the histopathological changes showed ameliorative effects of roflumilast in compared
to glycerol group. From this study, one could be concluded the protective effect of roflumilast in case of
renal failure induction by glycerol.