Validation of Horizontal Genes Transfer Using Selected Clinical Strain of P. Mirabsilis for Swarming Activity

Abstract

Introduction: Proteus mirabilis expresses several virulence factor which enable them to colonize, survive
and grow in the host organism. This study was to investigate whether P. mirabilis could deliver a spontaneous
plasmids to competent strains, and to investigate whether P. mirabilis can be as a donor strains to delivery
their virulent such as adhesion and swarming activity.
Methodology: PCR was used to detect the motility genes that encoded in P. mirabilis. Using also
transformation assay to transfer these genes of Proteus to standard strain. PCR technique used again to
determine the whether genes were transfer or not after transformation.
The results: the findings show that 9 (filD, flhC, flhD, motA, motB, fliL, flaA, flgM,) genes were encoded
on chromosomal DNA of P. mirabilis. Interestingly data, only 3 (flaA, flgM and filD ) genes were carried
on different plasmids of P. mirabilis. The results also show that spontaneous plasmids of P. mirabilis can
transfer to standard strains of E. coli. We found one gene transfer to E. coli S17? filD. The gene(filD) has a
role in the P. mirabilis swarming motility.
Conclusions: we demonstrated that these genes can carry not only on chromosomal DNA but also in
Plasmidal DNA and transferring genes associated-characteristics, swarming motility