CYP2D6 (G1934A) Gene Polymorphism in Rice Farmers with Long-term Pesticide Exposure, Suphan Buri, Thailand

Abstract

CYP2D6 G1934A (rs 3892097) gene polymorphism in coding region is leads to catalytic rate of cytochrome
P450 enzyme activity toward xenobiotic, including insecticide. Study was aimed to investigate CYP2D6
(G1934A) gene polymorphism in rice farmers along with occupational health risks and behaviors; and to
evaluate the relationship of gene polymorphisms and serum cholinesterase (SChE) levels. Cross-sectional
study was carried out from June 2019 to February 2020. Data had collected from health service program
by U-Thong district health promoting hospital. Gathered information concerning of pesticide exposures
from 50 rice farmers and 50 control respondents was conducted by questionnaire interviewing. Each
serum sample was determined SChE activity test by paper test and automatic analyzer; and EDTA blood
was analyzed genotyping by PCR-RFLP. Chi-square was used to analyze on the different of personal
information; and of polymorphism. Person correlation was evaluated relationship between SChE level and
genotypes. 60% of rice farmers were used pesticide over 10 years. Means of SChE level of both groups
were within reference value and significantly different (p = 0.033). Polymorphisms were included wild type
(GG), heterozygote (GA) and homozygote (AA); and were significantly different between rice farmers and
control (p = 0.0001). GA genotype in rice farmers was more frequent and AA genotype was not detected.
Relationship of polymorphism and SChE level were negative correlated (r = -0.258; p = 0.009). CYP2D6
gene polymorphism may useful biomarker for duties assignments in agricultural workers especially risk
group (GA genotype).