Patterns of Antimicrobial Resistance on Staphylococcus Aureus Isolated From Raw Milk from Dairy Farms in Surabaya

Abstract

The aim of this research was to show the pattern of S. aureus bacteria isolated from raw milk from two dairy
farms in Surabaya against antibiotics oxacillin, penicillin, amphicillin, chloramphenicol, and gentamicin.
The study used purposive sampling method. About 72 samples isolated on Mannitol Salt Agar media (MSA)
from the result which identified as Staphylococcus sp was 14 (19.44%) showed the positive samples on
coagulase test. Antibiotic disk used i.e oxacillin, penicillin, amphicillin, chloramphenicol, gentamicin by
using Kirby Bauer method. Inhibiton diameter zone measured at millimeter to determined a sensitivity level
of antibiotic. The result showed about 14 (100%) of samples was resistant to Antibiotic oxacillin type, 14
(100%) of samples was resistant to penicillin, 9 (64,2%) samples was resistant, and 5 (35,7%) of samples
was sensitive to amphicillin, 14 (100%) of samples was sensitive to chloramphenicol, and 14 (100%) of
samples was sensitive to gentamicin. These results will open up our view that cooking milk is important to
eliminate the danger of S. aureus isolates which present in milk and have potential hazards to public health.