Effect of Acridine Orange on Pathogenicity of E. coli Isolated from Urinary Tract Infection Patients

Abstract

A total of (108) isolate from Urinary tract infection (UTI) patients, only (47) isolate gave a positive result
(43.51%) after a number of morphological and biochemical characterization. Our result showed only 28
isolate have the ability for protease production and haemolysin production (59.5%), and E. coli no.6 was
the highest protease production isolate (24mm of lysis area) while the E. coli no.18 was lowest protease
production (12 mm of lysis area). After incubtion on skim milk agar medium at 37°C for 18h and with
(100%) haemolysin activity for all 28 isolates after growing on blood agar medium. In our study, first
attempt made on the effect of acridine orange (0.1%) as a curing agent on virulence factors of pathogenic
bacteria ( both protease and haemolysin productin) by highly producer E. coli no.6 at concentrations ( 0, 10-1
to 10-10) and the results showed that E. coli no.6 loss the protease and haemolysin productin at concentration
of (10-1 to 10-4) while little and normal activities were observed at concentration of (10-5 to 10-10) of acridine
orange. Also, this study was investigate the agarose - gel electrophoresis of both cured and normal cells and
the results showed the presence of both chromosomal and plasmid bands in the normal cells and only the
presence of chromosomal band for the cured cells E. coli treated with acridine orange at concentrations of
(10-2 to 10-4).