Exploring the Etiological Evidences at Avascular Necrosis of Femoral Head by Investigative Approaches of Histopathology, Clinical Assessment and Radiology

Abstract

Background- The avascular necrosis of the femoral head includes interference of the blood flow to the
femoral head. This ischemia leads to death of the bone cells and bone marrow followed by the collapse of
the bone femoral head, which finally leads to the destruction of the bone tissue and arthritis,
Objectives:
1. Histopathological analysis of the tissue obtained after core biopsy or sections of head after head excision
while doing Arthroplasty.
2. Correlation between histopathological evidence with clinical and radiological findings.
Methodology: The tissue will be sampled from site of AVN during surgical procedure that is Core
decompression or hip arthroplasty. Tissue material obtained will be transfered to regular histological fixative
of 10 % formalin. It will be fixed for a day, later to be reprocessed for decalcification. Formalin fixed tissue
will be subjected for treatment of running water to remove excess of formalin. Later the tissue will be
examined for its gross appearance of any changes of autolysis The process of decalcification adopted for
present work is chemical one. The decalcification tissue will be subjected for histokinette cycle. The tissue
is now ready for paraffin block. These paraffin blocks will be subjected to microtomy for obtaining 5 micro
milimeter sections. These paraffin sections will be stained for H/E and Van. Giesson stain. Histopathological
assessment of the tissue will be carried out for the evidence and grading of avascular necrosis.
Expected Results: Correlation between histopathological evidence with clinical and radiological findings.
Conclusion: This study will be helpful in developing Histopathological staging in correlation with clinical,
radiological findings.