Development and Standardization of Dental Plant-Based Preparation in The Republic of Uzbekistan

Abstract

Nowadays herbal medicines are widely used in official medical practice in Uzbekistan. At the same time,
the most common among both imported and domestic plant-based preparations are liquid dosage forms.
The modern stage of application of these medicines is based on the rational use not only of the accumulated
experience, but also on the achievements of scientific medicine in the study of the composition and features
of biological activity of medicinal plants and isolated from them active compounds. Studies have shown that
these drugs contain unique in composition combinations of biologically active substances, providing a wide
range of therapeutic and preventive action. To solve the problem developed the composition and obtained
on its basis a liquid extract for the treatment of diseases of the oral cavity of the mixture of plants such as
chamomile drug, sage drug, St. John’s wort, calendula drug in the ratio of raw materials - extraction fluid
1:1, established the most optimal extraction fluid - 70% ethyl alcohol and obtained alcohol extraction.
The resulting liquid extract was conditionally called “Parodonfit” and analyzed according to the
requirements of regulatory documentation. Each of the plants has its own quality standard. Qualitative
and quantitative analyses of biologically active substances of liquid extract “Parodonfit” with the use of
physical and chemical method, such as thin layer chromatography, chromatography spectrophotometry, gasliquid
chromatography was carried out. Biologically active substances in the liquid extract - flavonoids
and tannins - were determined during the research. The sum of flavonoids was determined by TLC, where
compounds of routine, quercetin, cinarozid were isolated. Qualitative analysis of tannins and determination
of heavy metals were conducted by pharmacopoeia method. Quantitative determination of active substances
of cinarozid by chromatospectrophotometry was carried out. The tannins in recalculation for tannin - 3,2
% and in recalculation for proanthocyanides - 6,38 % were found out by chemical reactions according to
the GD (governing documents). Quality indicators for liquid extract “Parodonfit” have been developed
according to the requirements of the normative document: physical and chemical indicators, ethyl alcohol
concentration, heavy metals, microbiological purity. The received results have shown perspective and
expediency of development of highly effective multicomponent medical products on the basis of medicinal
vegetative raw materials.