Histopathological Assessment of Autopsied Salivary Gland Tissue to Estimate the Post Mortem Interval – A Cross Sectional Observational Study
Keywords:autopsy, histopathology, oral tissue, post-mortem interval, salivary gland,
Background: In biological system, cells are considered as dynamic and complex structures likewise cellular
disintegration is also a complex process which occurs in a sequence. Therefore, these autolytic cellular
changes have been investigated by forensic pathologist in an attempt to find markers that may assist in
determining the time of death. In this study we attempt to analyse the efficacy of oral soft tissues specifically
salivary gland in concluding the time since death.
Methodology: After obtaining approval from the human ethical committee, informed consent from the
relative of the corpse and the investigating police officer, demographic information, as well as post mortem
number was recorded. Submandibular Salivary Gland was collected for histopathological analysis during
routine autopsy procedure. The specimens were fixed immediately in 10% formalin, processed, sectioned
and stained with haematoxylin and eosin. The stained sections were evaluated under light microscope for
Conclusion. The histopathological changes in the autopsied salivary gland tissue can be used as an adjuvant
to estimate the post-mortem interval.
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