Study the Role of Ph in Curli Biogenesis Gene Expression in Enterobacter Cloacae Local Isolates

Abstract

Enterobacter cloacae is the most common pathogenic species of the genus Enterobacter, identified in
hospitalized and enfeebled patients as the etiologic agent of many infections and considered as a significant
bacterial pathogen in recent years. E. Cloacae are typical gram negative opportunistic forms of bacteria that
cause illness after another infection or injury has influenced the host immune system and are related with
nosocomial infections. The infection may be caused by GIT, UTI or cross contamination of the blood. The
present study focused on the existence of the main curli biogenesis gene (csgA) and the role of pH as an
environmental factor in the gene expression (csgA) for curli biogenesis. In this study, urine samples from
75 patients.In Baghdad city clinically diagnosed with urinary tract infection,for gram staining, Api20System
and gene expression (csgA) gene in Standardized pH7 and acid pH4 RT-PCR was performed with specific
primers. Conventional gram staining techniques, agricultural methods and System API 20E showed good
results for E. Cloacae. For the 75 patients, 10 (13.3%) the findings find the highest gene expression fold
values for the (csgA)gene in pH7 while the lowest fold value for (csgA) at acid pH4 (0.1577) thus the
modify conditions growth such as pH of bacteria E. Cloacae triggers gene expression changes for biogenesis
process. the findings of rpoB gene expression, which was used as a control gene, indicated that this gene was
good as a housekeeping gene.