Study of Sperm Dna Fragmentation Index in Patients from Rural Areas of Wardha Region

Authors

  • Kanchi S. Goenka
  • Akash More
  • Mehul Salve
  • Deepti Shrivastava

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14300

Keywords:

Sperm DNA fragmentation (SDF), DNA fragmentation index (DFI), oxidative stress, genetic abnormalities, hyperthermia, gonadotoxins and endocrine abnormalities.

Abstract

Background: Sperm DNA fragmentation test is an advanced test for proper sperm function with respect
to semen analysis, as semen analysis is used for motility and morphology assessment, it cannot explain
sperm at molecular level and thus SDF test has been introduced to evaluate male fertility. The integrity of
genetic material in sperm is crucial for successful fertilization & normal embryo development as this will
lead to proper transmission of genetic information. Patients having varicocele, recurrent failure of ART
tecniques and improper lifestyle (radiation, smoking, alcohol, BMI, ageing, etc) are considered for sperm
DNA fragmentation test. SDF test is useful in selecting patients for the ART procedures i.e. IVF/ICSI/IUI
and highest pregnancy and live birth outcomes.
Objectives:
· To predict male infertility with normal semen parameters & its diagnostic values.
· To investigate the clinical factors related to sperm DNA fragmentation in male with infertility.
· To investigate the rate of sperm DNA fragmentation in male related to improper fertilization, embryo
development and recurrent pregnancy loss.
Methods: The participants (n=40) with male fertility problems will be included in randomised control trial.
All 40 participants will be randomly selected to evaluate their sperm DNA integrity from their respective
semen samples using sperm chromatin dispersion test.
Discussion: Efficiency of the Sperm DNA Fragmentation test is assessed by performing sperm chromatin
dispersion test on respective processed semen sample using Halosperm G2 test kit.

Author Biographies

Kanchi S. Goenka

M. Sc. Clinical Embryology, School Of Allied Health Sciences, Datta Meghe Institute Of Medical Sciences,
Wardha, Maharashtra, India

Akash More

Senior Embryologist, Wardha Test tube Baby Centre, Datta Meghe Institute of
Medical science, Sawangi (Meghe), Wardha, Maharashtra, India

Mehul Salve

Associate Professor, Department of OBGY,
Jawaharlal Nehru Medical College, Sawangi (Meghe), Wardha, Maharashtra, India

Deepti Shrivastava

Professor, department of
OBGY, director of Wardha Test tube Baby Centre, Jawaharlal Nehru Medical College, Sawangi (Meghe), Wardha,
Maharashtra, India

Published

2021-03-24

How to Cite

Kanchi S. Goenka, Akash More, Mehul Salve, & Deepti Shrivastava. (2021). Study of Sperm Dna Fragmentation Index in Patients from Rural Areas of Wardha Region. Indian Journal of Forensic Medicine & Toxicology, 15(2), 181-187. https://doi.org/10.37506/ijfmt.v15i2.14300