Estimation of Chronological Age from Advanced Glycation End Products Level in Vitreous Fluid

Authors

  • Kanicnan Intui
  • Churdsak Jaikang
  • Somlada Watcharakhom
  • Yutti Amornlertwatana

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14301

Keywords:

Age estimation, advanced glycation end products, vitreous humor, pentosidine

Abstract

Background: Chronological age estimation is an important process for corpse identification. Advanced
glycation end products (AGEs) are accumulated during lifetime and have been used for forensic identification.
The AGEs in vitreous humor still lack of information for age estimation. Objective: To investigate correlation
between the AGEs level contained in vitreous humor and chronological age in postmortem cases. Methods:
The vitreous humor samples were collected from the postmortem cases (n=142). The AGEs levels were
determined by spectrofluorometer and presented in microgram of quinidine hemisulfate equivalent (?g QE/
mL) and nanogram of pentosidine equivalent (ng PE/mL). Stepwise linear regression was used to generate
the equation. Results: The QE and PE showed mild positive correlation (r = 0.350 and 0.195; p <0.001,
respectively) while the PE/QE ratio showed moderate negative correlation with age (r = -0.561, p <0.001).
The predicted equation showed error value 12 years and gave accurate prediction in range 40-59 years old.
Conclusion: The AGEs contained in the vitreous humor correlated with chronological age in postmortem
cases. The other fluorescence molecules might be interfere during the AGEs measurement then the specific
methods should be developed for measuring the AGEs level to decrease the error value.

Author Biographies

Kanicnan Intui

Resident training

Churdsak Jaikang

Assistant Professor, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai
University, Chiang Mai, Thailand

Somlada Watcharakhom

Resident training

Yutti Amornlertwatana

Assistant Professor, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai
University, Chiang Mai, Thailand

Published

2021-03-24

How to Cite

Kanicnan Intui, Churdsak Jaikang, Somlada Watcharakhom, & Yutti Amornlertwatana. (2021). Estimation of Chronological Age from Advanced Glycation End Products Level in Vitreous Fluid. Indian Journal of Forensic Medicine & Toxicology, 15(2), 188-194. https://doi.org/10.37506/ijfmt.v15i2.14301