Molecular Identification of T. mentagrophytes and M. canis Isolated from Children Infected with Tinea Capitis in Basra Governorate by Using AP-PCR Technique
Keywords:tinea capitis , dermatophytes , molecular identification
The current study included molecular identification of dermatophytes causing Tinea capitis in children in
Basrah governorate, including T. Mentagrophytes&M. canis, using AP-PCR or RAPD –PCR polymerase
chain reaction technique. By using EZ-10 Spin Column Fungal Genomic DNA Mini –Preps Kit used to
extract DNA from dermatophytes fungi, where DNA was obtained from these two dermatophytes fungi.
The DNA bands appeared clearly after the electrophoresis of the DNA extract showed the results of DNA
The efficiency of the primeres used in the diagnosis of these two types of dermatophytes, called: random
primeres OPAA11, OPAA17, OPU15, OPD18, was obtained by the positive results obtained when amplified
using these primeres.Used for both dermatophytes The results obtained by using OPAA17 showed success
in DNA amplification - obtained from M.canis and T. Mentagrophytes, with six bands for M.canis. As for
T. Mentagrophytes, the number of bands obtained was 13, and the amplification results obtained using
OPAA11 showed the efficiency of this primer.
Five bands were obtained when used to amplify the DNA of the dermatophytes. While seven bands belonged
to T. Mentagrophytes, The amplification results of OPU15 indicated that there were six bands for M.canis,
while only one bande for T. Mentagrophytes showed the results of amplification using OPD18 used in
diagnosis of dermatophytes to four bands for M.canis while the number of bands for T. Mentagrophytes has
ten bands. This method for identifecation is fast and has a high diagnostic efficiency of dermatophytes, as
the diagnosis based on genetic characteristics is more accurate than resulting on phenotypic characteristics .
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions