Molecular Identification of T. mentagrophytes and M. canis Isolated from Children Infected with Tinea Capitis in Basra Governorate by Using AP-PCR Technique

Authors

  • Shrouk A. AL-Ibraheem
  • Nasir A. AL-Mansour
  • Kadhim k.AL-Rubaiee

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14503

Keywords:

tinea capitis , dermatophytes , molecular identification

Abstract

The current study included molecular identification of dermatophytes causing Tinea capitis in children in
Basrah governorate, including T. Mentagrophytes&M. canis, using AP-PCR or RAPD –PCR polymerase
chain reaction technique. By using EZ-10 Spin Column Fungal Genomic DNA Mini –Preps Kit used to
extract DNA from dermatophytes fungi, where DNA was obtained from these two dermatophytes fungi.
The DNA bands appeared clearly after the electrophoresis of the DNA extract showed the results of DNA
amplification .
The efficiency of the primeres used in the diagnosis of these two types of dermatophytes, called: random
primeres OPAA11, OPAA17, OPU15, OPD18, was obtained by the positive results obtained when amplified
using these primeres.Used for both dermatophytes The results obtained by using OPAA17 showed success
in DNA amplification - obtained from M.canis and T. Mentagrophytes, with six bands for M.canis. As for
T. Mentagrophytes, the number of bands obtained was 13, and the amplification results obtained using
OPAA11 showed the efficiency of this primer.
Five bands were obtained when used to amplify the DNA of the dermatophytes. While seven bands belonged
to T. Mentagrophytes, The amplification results of OPU15 indicated that there were six bands for M.canis,
while only one bande for T. Mentagrophytes showed the results of amplification using OPD18 used in
diagnosis of dermatophytes to four bands for M.canis while the number of bands for T. Mentagrophytes has
ten bands. This method for identifecation is fast and has a high diagnostic efficiency of dermatophytes, as
the diagnosis based on genetic characteristics is more accurate than resulting on phenotypic characteristics .

Author Biographies

Shrouk A. AL-Ibraheem

Lect. Southern Technical University / College of Health and Medical Technology/Iraq

Nasir A. AL-Mansour

Prof. Basrah University/ College of Science/ Iraq

Kadhim k.AL-Rubaiee

Prof. Basrah University/ College of Science/ Iraq

Published

2021-03-24

How to Cite

Shrouk A. AL-Ibraheem, Nasir A. AL-Mansour, & Kadhim k.AL-Rubaiee. (2021). Molecular Identification of T. mentagrophytes and M. canis Isolated from Children Infected with Tinea Capitis in Basra Governorate by Using AP-PCR Technique. Indian Journal of Forensic Medicine & Toxicology, 15(2), 1279-1285. https://doi.org/10.37506/ijfmt.v15i2.14503