Study Some Kinetic Parameters for Purified Arginase from Two Different Sources: Red Blood Cells and Serum of( Healthy and TypeII Diabetic Patient)
Keywords:Arginase , diabetes mellitus, kinetic parameters, Atrovastatin, Arginase regulatory .
Kinetics parameters for arginase that purified from RBCs, and serum (control, and diabetic patients)
revealed that the Km values are (200, 416, and 333mM), Vmax values are (47, 5.5, and 9 U/min),and
The values of catalytic constant Kcat are (174, 45.8, and 81.8 min-1). The effect of atrovastatin was also
studied on serum arginase, the arginase activity is decreasing by increasing statin concentration, wherein
at the lower concentration (0.112 mg/ml) the allosteric regulation percentage is 26% and it is ongoing until
reaching its maximum (85%) at the highest concentration (0.9mg/ml). In conclusion affinity of arginase to
the L-arginine, and its velocity is more in RBCs, and serum patients compared with serum controls, and
statin appears to be an allosteric regulator for arginase.
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