Study Some Kinetic Parameters for Purified Arginase from Two Different Sources: Red Blood Cells and Serum of( Healthy and TypeII Diabetic Patient)

Authors

  • Fayhaa M. Khaleel
  • Essam F.A.Al-Jumaili

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14551

Keywords:

Arginase , diabetes mellitus, kinetic parameters, Atrovastatin, Arginase regulatory .

Abstract

Kinetics parameters for arginase that purified from RBCs, and serum (control, and diabetic patients)
revealed that the Km values are (200, 416, and 333mM), Vmax values are (47, 5.5, and 9 U/min),and
The values of catalytic constant Kcat are (174, 45.8, and 81.8 min-1). The effect of atrovastatin was also
studied on serum arginase, the arginase activity is decreasing by increasing statin concentration, wherein
at the lower concentration (0.112 mg/ml) the allosteric regulation percentage is 26% and it is ongoing until
reaching its maximum (85%) at the highest concentration (0.9mg/ml). In conclusion affinity of arginase to
the L-arginine, and its velocity is more in RBCs, and serum patients compared with serum controls, and
statin appears to be an allosteric regulator for arginase.

Author Biographies

Fayhaa M. Khaleel

Assist. Prof., Chemistry Dept. College of Science for Women, University of Baghdad, Baghdad, Iraq

Essam F.A.Al-Jumaili

Prof., Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq

Published

2021-03-24

How to Cite

Fayhaa M. Khaleel, & Essam F.A.Al-Jumaili. (2021). Study Some Kinetic Parameters for Purified Arginase from Two Different Sources: Red Blood Cells and Serum of( Healthy and TypeII Diabetic Patient). Indian Journal of Forensic Medicine & Toxicology, 15(2), 1503-1510. https://doi.org/10.37506/ijfmt.v15i2.14551