Molecular Study of Virulence Factors (Cysteine Protease-1 and Amoebapore-C) for Entamoeba histolytica in Babylon Province

Authors

  • Entessar Hatif Abd Al-Kathem Al-Murshidi
  • Ahmed Khudhair Al-Hamairy
  • Hadi Meziel Khudhair

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14560

Keywords:

Entamoeba histolytica, Cysteine Protease-1, Amoebapore-C

Abstract

A molecular study was conducted for the period from December 2019 to May 2020, which included
examining (50) positive stool samples by using the direct smear method. that was revealed by the method of
polymerase chain reaction (PCR) using the gene (18SrRNA), with a percentage of 82%, distributed 87.8%
for males and 70.6% for females. Then for determine the virulence factors ( Cysteine proteinase-1) and
(Amoebapores-C) by using primers, with (PCR)technique , and then electrophoresis on the agarose gel have
been done , the results were positive for Cysteine proteinase-1 (APC-1) at a wavelength (212 bp) and the
Amoebapore-C (Amo-C) at a wavelength (536 bp). Five random positive samples of the PCR product in the
current study were sent to Macrogen in South Korea for the purpose of determining the DNA sequence of
the amoebapore- C gene present in the Entamoeba histolytica, and it is the first study in Iraq and Babylon
province that is genetically determined by the NCBI These genotypes of the amoebic holes gene are recorded
in the gene bank. The results of the study showed that all the transmitted isolates are identical with NCBI
isolates with the USA samples registered with the gene bank with accession number AY956434.2, which
are the isolated samples belonging to the American samples and diagnosed with the number MS30-1043 of
the USA study by the researcher Bhattacharya et al. (2005) with the current study samples As it was 100%
identical. These samples were recorded in the genebank for the first time for this Amoebapore C gene under
Accession No. For samples (1, 2, 3, 4, 5) in sequences are MT951203, MT951204, MT951205, MT951206
and MT951207, respectively.

Author Biographies

Entessar Hatif Abd Al-Kathem Al-Murshidi

M.Sc. Student

Ahmed Khudhair Al-Hamairy

Prof. Dr., College of Science for Women, University of Babylon, Hilla, Iraq

Hadi Meziel Khudhair

Prof. Dr., College of Science for Women, University of Babylon, Hilla, Iraq

Published

2021-03-24

How to Cite

Entessar Hatif Abd Al-Kathem Al-Murshidi, Ahmed Khudhair Al-Hamairy, & Hadi Meziel Khudhair. (2021). Molecular Study of Virulence Factors (Cysteine Protease-1 and Amoebapore-C) for Entamoeba histolytica in Babylon Province . Indian Journal of Forensic Medicine & Toxicology, 15(2), 1565-1574. https://doi.org/10.37506/ijfmt.v15i2.14560