Phenotypic and Molecular Detection of Pyocin from Multidrug-Resistant Pseudomonas aeruginosa Isolated from Various Pathogenic

Authors

  • Ahmed Yas Saeed
  • Dhyaa Fandi Ahmed
  • Mahmood Khlaf Saleh
  • Adnan F. Alazzawie
  • Mostafa Qahtan Mostafa
  • Fatima Mustafa Mahdii
  • Nedaa Wasmi Shehab
  • Omar A.Mohammed Al Hashimi

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14577

Keywords:

Pseudomonas aeruginosa, Pyocin , PrtN.

Abstract

This study included collecting (114) pure isolates of Pseudomonas aeruginosa, distributed over wounds
(39) isolates, burns (31) isolates, urinary tract infections (23) isolations, ear infections (12) isolations, and
respiratory infections (9) Isolates. The test for the sensitivity of the bacteria to (14) types of antibiotics
showed a clear variation in their resistance to antibiotics, as all isolates showed 100% resistance to
Ampicillin and Novabocin, Nalidixic acid by 93.8%, and Bacitracin by 87.7%, while they had low resistance
to Ciprofloxacin, 21%, Cefotaxime 19.2%, and Chloramphenicol 18.4%. While the results of the phenotypic
detection of pyocin showed the presence of (85 isolates, at a rate of 74.5%), producing Pyocin, while (29
isolates, at a rate of 25.4%), it showed its inability to produce Pyosin. As for the molecular detection of the
PrtN gene, which is one of the most important genes responsible for the production of Pyocin, It was shown
that (17 isolates, 77.2%) possess this gene, while (5 isolates, at a rate of 22.7%), they did not possess this
gene.

Author Biographies

Ahmed Yas Saeed

Stdnt., Education Department-Salah El Din Education Directorate-Ministry of Education

Dhyaa Fandi Ahmed

MB. MB.CH.B.DH.
Surgery

Mahmood Khlaf Saleh

Asst.Prof. Department of Biology, Education of Pure sciences college, University of Tikrit

Adnan F. Alazzawie

Prof.,
Department of Biology, College of Science, University of Tikrit

Mostafa Qahtan Mostafa

Lec. Department of Biology, College of Science,
University of Tikrit

Fatima Mustafa Mahdii

Stdnt. Department of Biology, College of Science, University of Tikrit

Nedaa Wasmi Shehab

Asst. Lec. . Education
Department-Salah El Din Education Directorate-Ministry of Education

Omar A.Mohammed Al Hashimi

Stdnt. Qollege of Education and Pure
Sciences, Tikrit University

Published

2021-03-24

How to Cite

Ahmed Yas Saeed, Dhyaa Fandi Ahmed, Mahmood Khlaf Saleh, Adnan F. Alazzawie, Mostafa Qahtan Mostafa, Fatima Mustafa Mahdii, Nedaa Wasmi Shehab, & Omar A.Mohammed Al Hashimi. (2021). Phenotypic and Molecular Detection of Pyocin from Multidrug-Resistant Pseudomonas aeruginosa Isolated from Various Pathogenic. Indian Journal of Forensic Medicine & Toxicology, 15(2), 1659-1667. https://doi.org/10.37506/ijfmt.v15i2.14577

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