Phenotypic and Molecular Detection of Pyocin from Multidrug-Resistant Pseudomonas aeruginosa Isolated from Various Pathogenic
Keywords:Pseudomonas aeruginosa, Pyocin , PrtN.
This study included collecting (114) pure isolates of Pseudomonas aeruginosa, distributed over wounds
(39) isolates, burns (31) isolates, urinary tract infections (23) isolations, ear infections (12) isolations, and
respiratory infections (9) Isolates. The test for the sensitivity of the bacteria to (14) types of antibiotics
showed a clear variation in their resistance to antibiotics, as all isolates showed 100% resistance to
Ampicillin and Novabocin, Nalidixic acid by 93.8%, and Bacitracin by 87.7%, while they had low resistance
to Ciprofloxacin, 21%, Cefotaxime 19.2%, and Chloramphenicol 18.4%. While the results of the phenotypic
detection of pyocin showed the presence of (85 isolates, at a rate of 74.5%), producing Pyocin, while (29
isolates, at a rate of 25.4%), it showed its inability to produce Pyosin. As for the molecular detection of the
PrtN gene, which is one of the most important genes responsible for the production of Pyocin, It was shown
that (17 isolates, 77.2%) possess this gene, while (5 isolates, at a rate of 22.7%), they did not possess this
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