Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells

Abstract

Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expression
and decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.
Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (rats
receiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil,
one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receiving
nanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, the
rats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. On
day 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured using
immunohistochemical methods.
Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. In
contrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-induced
rats ovarian granulosa cells.
Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SOD
and the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells.