Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells

Authors

  • Anis Satus Syarifah
  • Sri Agus Sudjarwo
  • Hendy Hendarto
  • Reny I’tishom
  • Supriyanto

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14650

Keywords:

Antioxidants; lead acetate; SOD and Caspase-3; nanocurcumin

Abstract

Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expression
and decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.
Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (rats
receiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil,
one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receiving
nanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, the
rats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. On
day 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured using
immunohistochemical methods.
Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. In
contrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-induced
rats ovarian granulosa cells.
Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SOD
and the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells.

Author Biographies

  • Anis Satus Syarifah

    Assistant Professor, Nursing Professional Education Program, STIKES Pemkab Jombang, Indonesia

  • Sri Agus Sudjarwo

    Professor,
    Department of Pharmacology, Faculty of Veterinary Medicine, Universitas, Airlangga, Surabaya, Indonesia

  • Hendy Hendarto

    Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas, Airlangga, Surabaya,
    Indonesia

  • Reny I’tishom

    Associate Professor, Department of Medical Biology, Faculty of Medicine, Universitas, Airlangga,
    Surabaya, Indonesia

  • Supriyanto

    Assistant Professor, Department of Nursing, Politehnik Kesehatan Kemenkes Surabaya,
    Indonesia

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Published

2021-03-24

How to Cite

Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells. (2021). Indian Journal of Forensic Medicine & Toxicology, 15(2), 1961-1969. https://doi.org/10.37506/ijfmt.v15i2.14650