Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells

Authors

  • Anis Satus Syarifah
  • Sri Agus Sudjarwo
  • Hendy Hendarto
  • Reny I’tishom
  • Supriyanto

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14650

Keywords:

Antioxidants; lead acetate; SOD and Caspase-3; nanocurcumin

Abstract

Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expression
and decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.
Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (rats
receiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil,
one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receiving
nanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, the
rats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. On
day 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured using
immunohistochemical methods.
Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. In
contrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-induced
rats ovarian granulosa cells.
Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SOD
and the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells.

Author Biographies

Anis Satus Syarifah

Assistant Professor, Nursing Professional Education Program, STIKES Pemkab Jombang, Indonesia

Sri Agus Sudjarwo

Professor,
Department of Pharmacology, Faculty of Veterinary Medicine, Universitas, Airlangga, Surabaya, Indonesia

Hendy Hendarto

Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas, Airlangga, Surabaya,
Indonesia

Reny I’tishom

Associate Professor, Department of Medical Biology, Faculty of Medicine, Universitas, Airlangga,
Surabaya, Indonesia

Supriyanto

Assistant Professor, Department of Nursing, Politehnik Kesehatan Kemenkes Surabaya,
Indonesia

Published

2021-03-24

How to Cite

Anis Satus Syarifah, Sri Agus Sudjarwo, Hendy Hendarto, Reny I’tishom, & Supriyanto. (2021). Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells. Indian Journal of Forensic Medicine & Toxicology, 15(2), 1961-1969. https://doi.org/10.37506/ijfmt.v15i2.14650