Ketogenic Diet Slows Down Weight Gain in Juvenile Mus musculus with Benzopyrene as Cancer Inducer

Authors

  • Dita Mega Utami
  • Lilik Herawati
  • Reny I’tishom
  • Mohammad Anam Al-Ari
  • Muhammad Miftahussurur
  • Purwo Sri Rejeki

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14709

Keywords:

ketogenic diet, weight gain, benzopyrene induced, ketone, blood glucose

Abstract

Objective: This study aims to analyze the ketogenic diet that slows down weight gain in juvenile Mus
musculus induced with benzopyrene (BZP). Method: Thirty-six female mice (Mus musculus), 6-8 weeks old,
weighted 15-25 grams were divided into six groups randomly. K1 (negative control, standard diet) induced
with oleum olivarum, while K2 (positive control, standard diet), P1 (ketogenic diet 1), P2 (ketogenic diet 2),
P3 (ketogenic diet 3) and P4 (ketogenic diet 4) induced with BZP subcutaneously in the right breast at a dose
of 0.3mg/20gBB/day for 14 days. The ketogenic diet was given for the next 28 days. Data were analyzed
using the ANOVA and post hoc LSD test. Results: After 42 days, the ketogenic diet mice experienced
slower weight gain (p=0.000) because BZP induced neurotoxicity and the administration of the ketogenic
diet in juvenile mice induced neurohormonal changes. The ketogenic diet increased ketone plasma level
(p=0.000) and reduced blood glucose levels (p=0.002) due to nutritional ketosis state. Conclusions: The
ketogenic diet with fat:protein ratio 60:30 slows down weight gain, increased ketone levels and decreased
blood glucose levels in juvenile mice with BZP as cancer inducer.

Author Biographies

Dita Mega Utami

Student, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Lilik Herawati

Lecturer, Department of Physiology,
Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Reny I’tishom

Lecturer, Department of Medical Biology,
Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Mohammad Anam Al-Ari

Lecturer, Department of Animal Husbandry,
Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia

Muhammad Miftahussurur

Lecturer, Department of Internal
Medicine, Dr. Soetomo General Hospital, Surabaya, Indonesia

Purwo Sri Rejeki

Lecturer, Department of Physiology,
Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Published

2021-03-24

How to Cite

Dita Mega Utami, Lilik Herawati, Reny I’tishom, Mohammad Anam Al-Ari, Muhammad Miftahussurur, & Purwo Sri Rejeki. (2021). Ketogenic Diet Slows Down Weight Gain in Juvenile Mus musculus with Benzopyrene as Cancer Inducer . Indian Journal of Forensic Medicine & Toxicology, 15(2), 2268-2274. https://doi.org/10.37506/ijfmt.v15i2.14709