Molecular Detection of Toxoplasma gondii in Helix pomatia in Wasit Province Iraq

Authors

  • Duaa Abdul Khaliq AL-Quraishy
  • Altamemy A. K. Aakool

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14710

Keywords:

Toxoplasma Helix IRAQ real time PCR

Abstract

DNA of a total of 96 samples of (32) enteric, (32) gastric and (32) pod of Helix pomatia has been obtained
through the use of the Genomic DNA Extraction kit and tested by the use of the Real time PCR and specific
set of the primers and probe for the detection of the Toxoplasma gondii B1 gene. Six enteric samples and only
2 gastric samples have been positive while the others have been negative, that result indicates the presence
of the T. gondii in that snail species, and based upon those results infection can either be acquired from the
contaminated environment or as a result of consuming the grass that has been contaminated with parasite
oocysts. The negative result of pod samples may be due to the failure of filtration the infectious stages of
parasites into the organs that close contacts with environment or inability of the parasites in surviving in
those organs.

Author Biographies

Duaa Abdul Khaliq AL-Quraishy

M.Sc.

Altamemy A. K. Aakool

Ph.D., Department of Biology, College of Science, University of Wasit

Published

2021-03-24

How to Cite

Duaa Abdul Khaliq AL-Quraishy, & Altamemy A. K. Aakool. (2021). Molecular Detection of Toxoplasma gondii in Helix pomatia in Wasit Province Iraq . Indian Journal of Forensic Medicine & Toxicology, 15(2), 2275-2280. https://doi.org/10.37506/ijfmt.v15i2.14710