In-Vitro Differentiation Adipose-Derived Mesenchymal Stem Cells into Pancreatic Progenitor Cells
Keywords:diabetes, adipose-derived mesenchymal stem cells, Pdx1, insulin
Background: Adult stem cells are currently reliable sources of mesenchymal stem cells for regenerative
therapy, include diabetes mellitus. The aim of this study is to develop endocrine pancreatic progenitor cells
characterized by Pdx1 and insulin expression from rat adipose-derived mesenchymal stem cells using two
steps in-vitro differentiation.
Methods: In this experimental study, ADMSCs were isolated from rat adipose tissue and exposed to
insulinogenic differentiation medium containing nicotinamide, activin A and glucagon-like peptide-1 (GLP1). After induction, the existence of pancreatic progenitor cells (PPCs) was confirmed by immune-staining
assay of Pdx1 and insulin.
Results: After three weeks of in-vitro differentiation, expression of Pdx1 and insulin proteins showed up
as green in the immunofluorescence assay. Immunofluorescence intensity of Pdx1 was higher in PPCs than
in ADMSCs control (p<0.05). Immunofluorescence intensity of insulin was also higher in PPCs than in
ADMSCs control (p<0.05). Therefore, in-vitro differentiation was successful to develop PPCs from rat
Conclusion: This study has demonstrated the in-vitro differentiation of ADMSCs into PPCs that expressed
Pdx1 and insulin
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