Cardiac Fibrosis Attenuation by Chlorogenic Acid and Epigallocatechin-Gallate Mediated by Suppression of Galectin-3 Gene Expression and Collagen Deposition in Rat Metabolic Syndrome Model

Authors

  • Indah Nur Chomsy
  • M. Saifur Rohman
  • Mifetika Lukitasari
  • Dwi Adi Nugroho
  • Husnul Khotimah

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14759

Keywords:

metabolic syndrome, chlorogenic acid, collagen deposition, epigalocathecin-gallate, galectin-3.

Abstract

Objective: Metabolic syndrome (MetS) is a set cluster of risk factors for metabolic abnormalities that
can develop cardiovascular disease (CVD), one of that is remodeling or cardiac fibrosis. Cardiac fibrosis
identified from the high levels of the profibrotic molecule; one of them is galectin-3. Aim: This study
intended to determine the effect of combination therapy of green coffee (CGA) and green tea (EGCG) on
cardiac fibrosis by raise of the galectin-3 gene expression and collagen deposition in rat cardiac tissue in
the rat metabolic syndrome model. Methods: Twenty-four male MetS rats (Sprague-Dawley) divided into
two control groups and three groups therapy (n=5) administration of the CGA 200mg/kgbw (body weight
in kilograms) and EGCG 300mg/kgbw orally. After eight weeks of treatment, rats euthanized, then mRNA
expression of galectin-3 was measured. Furthermore, collagen deposition of cardiac tissue carried out in
histology slides. Results: Research reveals that the expression of galectin-3 decreased in the metabolic
syndrome model group, which given combination therapy compared with metabolic syndrome model mice
that did not receive any therapy (P=0.000). Collagen deposition in cardiac tissue also found less than in
the therapy group compared with the group not treated with both compounds (P=0.000). The correlation
between the two parameters shows a positive association with low strength. Conclusion: This study shows
that the combination therapy of CGA and EGCG is an engaging therapeutic candidate. It expected to reduce
the progression of cardiac fibrosis in metabolic syndrome

Author Biographies

Indah Nur Chomsy

Master Program in Biomedical Sciences, Faculty of Medicine, University of Brawijaya, Jl. Veteran, Malang
65145, East Java, Indonesia

M. Saifur Rohman

Department of Cardiology and Vascular Medicine, Saiful Anwar General Hospital,
Faculty of Medicine, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia

Mifetika Lukitasari

Departement
of Nursing, Faculty of Medicine, University of Brawijaya, Jl. Veteran, Malang 65145, Indonesia

Dwi Adi Nugroho

Brawijaya
Cardiovascular Research Group, Faculty of Medicine, University of Brawijaya, Malang, Indonesia

Husnul Khotimah

Laboratory of
Pharmacology, Medical Faculty, Brawijaya University, Jl. Veteran, Malang 65145, Indonesia

Published

2021-03-24

How to Cite

Indah Nur Chomsy, M. Saifur Rohman, Mifetika Lukitasari, Dwi Adi Nugroho, & Husnul Khotimah. (2021). Cardiac Fibrosis Attenuation by Chlorogenic Acid and Epigallocatechin-Gallate Mediated by Suppression of Galectin-3 Gene Expression and Collagen Deposition in Rat Metabolic Syndrome Model. Indian Journal of Forensic Medicine & Toxicology, 15(2), 2567-2574. https://doi.org/10.37506/ijfmt.v15i2.14759