Effect of Extract and Caffeic Acid Phenethyl Ester on Apoptosis of Odontoblast Cells

Authors

  • Ira Widjiastuti
  • Agus Subiwahjudi
  • M. Aditya Syahputra
  • Grace Angelina Samuel
  • Ni Putu Sawitri Mega
  • Cinitra Anindya

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14765

Keywords:

odontoblast cell, apoptosis, propolis, caffeic acid phenetyl esters, herbal medicine

Abstract

Background: Propolis is a resinous hive product collected by bees from tree buds and mixed with secreted
bee wax to both avoid bacterial contamination in the hive and seal it. It is known to have a wide spectrum
of pharmacological properties, including anti-inflammatory, antioxidant, antibacterial, antiviral, and antifungal abilities. Apoptosis tests were performed on odontoblast cells in rats (Rattus novegicus) to assess
the viability of propolis extract and caffeic acid phenetyl esters (CAPE) as alternative candidates for pulp
capping agents in conservative dentistry treatment. Objective: To examine the apoptosis activity of propolis
extract and CAPE as capping materials on odontoblast cells. Methods: This study was designed as a posttest only control group laboratory experiment. The rats were randomly divided into three groups. Pulp
exposures were performed on the occlusal surface of the right maxillary first molars. In the first group, the
control group, glass ionomer cement (GIC) was directly applied to the pulp exposure. In the second group,
the sample group, propolis extract was applied to the pulp exposure, and in the third group, CAPE was
applied to the pulp exposure. All cavities were then filled with GIC as a permanent filling. Animals were
sacrificed on the first and fourteenth days. The direct counting method of histological examination was based
on the apoptotic odontoblasts, using the terminal deoxyribonucleotidyl transferase dUTP nick end labeling
(TUNEL) assay technique. Result: There was a greater number of apoptotic odontoblasts in the control
group, followed by the CAPE group and, lastly, the propolis extract group. Conclusion: The apoptosis
activity of the propolis extract is lower than that of CAPE.

Author Biographies

Ira Widjiastuti

Lecturer

Agus Subiwahjudi

Lecturer

M. Aditya Syahputra

Researcher & Postgraduate Student

Grace Angelina Samuel

Resident, Department of Conservative Dentistry and Endodontic,
Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Ni Putu Sawitri Mega

Resident, Department of Conservative Dentistry and Endodontic,
Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Cinitra Anindya

Resident, Department of Conservative Dentistry and Endodontic,
Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Published

2021-03-24

How to Cite

Ira Widjiastuti, Agus Subiwahjudi, M. Aditya Syahputra, Grace Angelina Samuel, Ni Putu Sawitri Mega, & Cinitra Anindya. (2021). Effect of Extract and Caffeic Acid Phenethyl Ester on Apoptosis of Odontoblast Cells. Indian Journal of Forensic Medicine & Toxicology, 15(2), 2606-2613. https://doi.org/10.37506/ijfmt.v15i2.14765